Создание и разработка лекарственных препаратов
Drug discovery and development
Программа магистратуры Медицинского института РУДН
/ Покровский Вадим
Покровский Вадим

Покровский Вадим

  • Доктор медицинских наук (онкология, биохимия)
  • Заведующий кафедрой биохимии им. Т.Т. Березова, руководитель программы
  • Заведующий лабораторией комбинированной терапии опухолей НМИЦ им. Н.Н. Блохина МЗ РФ
  • Аналитик, старший аналитик ООО «Биофармацевтические инвестиции РВК» (2011-2013)
  • Руководитель отдела стратегического развития ООО «Нанолек» (2013-2018)
  • Член рабочей группы Министерства образования и науки РФ по рассмотрению заявок по мероприятиям 5 «Доклинические исследования инновационных лекарственных средств» и 22 «Разработка новых образовательных программ» в рамках ФЦП «Фарма-2020» (2012-2018)
  • Член редакционной коллегии журналов Pharmaceuticals, Current Medicinal Chemistry
  • Сфера профессиональных интересов: изучение механизмов действия и антипролиферативной активности противоопухолевых препаратов, структурирование лицензионных сделок
Основные публикации

1) Plyasova AA, Berrino E, Khan II, Veselovsky AV, Pokrovsky VS, Angeli A, Ferraroni M, Supuran CT, Pokrovskaya MV, Alexandrova SS, Gladilina YA, Sokolov NN, Hilal A, Carta F, Zhdanov DD. Mechanisms of the antiproliferative and antitumor activity of novel telomerase-carbonic anhydrase dual-hybrid Inhibitors / J Med Chem. 2021. Vol. 64(15). P. 11432-11444. doi: 10.1021/acs.jmedchem.1c00756.

2) Lukasheva EV, Babayeva G, Karshieva SS, Zhdanov DD, Pokrovsky VS. L-lysine alpha-oxidase: enzyme with anticancer properties / Pharmaceuticals. 2021. Vol. 14(11):1070. doi:10.3390/ph14111070

3) Machulkin AE, Shafikov RR, Uspenskaya AA, Petrov SA, Ber AP, Skvortsov DA, Nimenko EA, Zyk NU, Smirnova GB, Pokrovsky VS, Abakumov MA, Saltykova IV, Akhmirov RT, Garanina AS, Polshakov VI, Saveliev OY, Ivanenkov YA, Aladinskaya AV, Finko AV, Yamansarov EU, Krasnovskaya OO, Erofeev AS, Gorelkin PV, Dontsova OA, Beloglazkina EK, Zyk NV, Khazanova ES, Majouga AG. Synthesis and biological evaluation of PSMA ligands with aromatic residues and fluorescent conjugates based on them / J Med Chem. 2021. Vol. 64(8). P. 4532-4552. doi: 10.1021/acs.jmedchem.0c01935.

4) Pokrovsky VS, Lukashev AN, Babayeva G, Karshieva SS, Arinbasarova AY, Medentzev AG, Komarova MV, Lukasheva EV. Plasma pharmacokinetics and tissue distribution of L-lysine α-oxidase from Trichoderma cf. aureoviride RIFAI VKM F- 4268D in mice / Amino Acids. 2021. Vol. 53(1). P. 111-118. doi: 10.1007/s00726-020-02930-4

5) Machulkin AE, Uspenskaya AA, Zyk NY, Nimenko EA, Ber AP, Petrov SA, Shafikov RR, Skvortsov DA, Smirnova GB, Borisova YA, Pokrovsky VS, Kolmogorov VS, Vaneev AN, Ivanenkov YA, Khudyakov AD, Kovalev SV, Erofeev AS, Gorelkin PV, Beloglazkina EK, Zyk NV, Khazanova ES, Majouga AG. PSMA-targeted small-molecule docetaxel conjugate: synthesis and preclinical evaluation / Eur J Med Chem. 2021. Vol. 227:113936. doi: 10.1016/j.ejmech.2021.113936.

6) Khan II, Karshieva SS, Sokolova DV, Spirina TS, Zolottsev VA, Latysheva AS, Anisimova NY, Komarova MV, Yakunina MN, Nitetskaya TA, Misharin AY, Pokrovsky VS. Antiproliferative, proapoptotic, and tumor-suppressing effects of the novel anticancer agent alsevirone in prostate cancer cells and xenografts / Arch Pharm. 2021:e2100316. doi: 10.1002/ardp.202100316.

7) Latysheva AS, Zolottsev VA, Pokrovsky VS, Khan II, Misharin AY. Novel nitrogen containing steroid derivatives for prostate cancer treatment / Current Medicinal Chemistry. 2021. doi: 10.2174/0929867328666210208113919.

8) Upadhyay N, Tilekar K, Loiodice F, Anisimova NY, Spirina TS, Sokolova DV, Smirnova GB, Choe JY, Meyer-Almes FJ, Pokrovsky VS, Lavecchia A, Ramaa CS. Pharmacophore hybridization approach to discover novel pyrazoline-based hydantoin analogs with anti-tumor efficacy / Bioorg Chem. 2021. Vol. 107:104527. doi: 10.1016/j.bioorg.2020.104527.

9) Zolottsev VA, Latysheva АS, Pokrovsky VS, Khan II, Misharin AY. Promising applications of steroid сonjugates for cancer research and treatment / Eur J Med Chem. 2021. Vol. 210:113089. doi: 10.1016/j.ejmech.2020.113089.

10) Plyasova AA, Pokrovskaya MV, Lisitsyna OM, Pokrovsky VS, Alexandrova SS, Hilal A, Sokolov NN, Zhdanov DD. Penetration into cancer cells via clathrin-dependent mechanism allows L-asparaginase from Rhodospirillum rubrum to inhibit telomerase / Pharmaceuticals. 2020. Vol. 13(10):286. doi: 10.3390/ph13100286.

11) Tilekar K, Upadhyay N, Meyer-Almes FJ, Loiodice F, Anisimova NY, Spirina TS, Sokolova DV, Smirnova GB, Choe JY, Pokrovsky VS, Lavecchia A, S Ramaa C. Synthesis and biological evaluation of pyrazoline and pyrrolidine-2,5-dione hybrids as potential antitumor agents / ChemMedChem. 2020. Vol. 15(19). P. 1813-1825. doi: 10.1002/cmdc.202000458.

12) Zhdanov DD, Plyasova AA, Pokrovsky VS, Pokrovskaya MV, Alexandrova SS, Gladilina YA, Sokolov NN. Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of normal human CD4 + T-lymphocytes / Biochimie. 2020. Vol. 174. P. 34-43. doi: 10.1016/j.biochi.2020.04.009

13) Latysheva AS, Zolottsev VA, Veselovsky AV, Scherbakov KA, Morozevich GE, Pokrovsky VS, Novikov RA, Timofeev VP, Tkachev YV, Misharin AY. New steroidal oxazolines, benzoxazoles and benzimidazoles related to abiraterone and galeterone / Steroids. 2020. Vol. 153. Article 108534. P. 1-9. doi: 10.1016/j.steroids.2019.108534

14) Zhdanov DD, Gladilina YA, Pokrovsky VS, Grishin DV, Grachev VA, Orlova VS, Pokrovskaya MV, Alexandrova SS, Plyasova AA, Sokolov NN. Endonuclease G modulates the alternative splicing of deoxyribonuclease 1 mRNA in human CD4+ T lymphocytes and prevents the progression of apoptosis / Biochimie; 2019; Vol. 157; 158-176. doi: 10.1016/j.biochi.2018.11.020

15) Zhdanov DD, Plyasova AA, Gladilina YA, Pokrovsky VS, Grishin DV, Grachev VA, Orlova VS, Pokrovskaya MV, Alexandrova SS, Lobaeva TA, Sokolov NN. Inhibition of telomerase activity by splice-switching oligonucleotides targeting the mRNA of the telomerase catalytic subunit affects proliferation of human CD4+ T lymphocytes / Biochemical and Biophysical Research Communications; 2019; Vol. 509(3); 790-796. doi: 10.1016/j.bbrc.2018.12.186

16) Pokrovsky VS, Vinnikov D. Defining the toxicity of current regimens for extranodal NK/T cell lymphoma: a systematic review and metaproportion / Expert Review of Anticancer Therapy. 2019; Vol. 19; N 1; 93-104. doi: 10.1080/14737140.2019.1549992

17) Pokrovsky VS, Chepikova OE, Davydov DZ, Zamyatnin AA, Lukashev AN, Lukasheva EV. Amino Acid Degrading Enzymes and Their Application in Cancer Therapy / Current Medicinal Chemistry; 2019; Vol. 26; N 3; 446-464. doi: 10.2174/0929867324666171006132729

18) Pokrovsky VS, Anisimova NYu, Davydov DZh, Bazhenov SV, Bulushova NV, Zavilgelsky GB, Kotova VY, Manukhov IV. Methionine gamma lyase from Clostridium sporogenes increases the anticancer effect of doxorubicin in A549 cells and human cancer xenografts / Investigational New Drugs; 2019; Vol. 37; N 2; 201-209. doi: 10.1007/s10637-018-0619-4

19) Zhdanov DD, Gladilina YA, Pokrovsky VS, Grishin DV, Grachev VA, Orlova VS, Pokrovskaya MV, Alexandrova SS, Sokolov NN. Murine regulatory T cells induce death of effector T, B, and NK lymphocytes through a contact-independent mechanism involving telomerase suppression and telomere-associated senescence / Cellular Immunology. 2018; Vol. 331; 146-160. doi: 10.1016/j.cellimm.2018.06.008

20) Zhdanov DD, Gladilina YA, Grishin DV, Grachev VA, Orlova VS, Pokrovskaya MV, Alexandrova SS, Pokrovsky VS, Sokolov NN. Contact-independent suppressive activity of regulatory T cells is associated with telomerase inhibition, telomere shortening and target lymphocyte apoptosis / Mol Immunol. 2018;101:229-244. doi: 10.1016/j.molimm.2018.07.017

21) Zhdanov DD, Pokrovsky VS, Pokrovskaya MV, Alexandrova SS, Eldarov MA, Grishin DV, Basharov MM, Gladilina YA, Podobed OV, Sokolov NN. Inhibition of telomerase activity and induction of apoptosis by Rhodospirillum rubrum L-asparaginase in cancer Jurkat cell line and normal human CD4+ Tlymphocytes / Cancer Med. 2017. doi: 10.1002/cam4.1218

22) Zhdanov DD, Pokrovsky VS, Pokrovskaya MV, Alexandrova SS, Eldarov MA, Grishin DV, Basharov MM, Gladilina YA, Podobed OV, Sokolov NN. Rhodospirillum rubrum L-asparaginase targets tumor growth by a dual mechanism involving telomerase inhibition / Biochem Biophys Res Commun. 2017; 492(2): 282-288. doi: 10.1016/j.bbrc.2017.08.078

23) Pokrovsky VS, Vinnikov D. L-Asparaginase for newly diagnosed extra-nodal NK/T-cell lymphoma: systematic review and meta-analysis / Expert Rev Anticancer Ther. 2017; 17(8):759-768. doi: 10.1080/14737140.2017.1344100

24) Morozova EA, Anufrieva NV, Davydov DZ, Komarova MV, Dyakov IN, Rodionov AN, Demidkina TV, Pokrovsky VS. Plasma methionine depletion and pharmacokinetic properties in mice of methionine γ-lyase from Citrobacter freundii, Clostridium tetani and Clostridium sporogenes / Biomed Pharmacother. 2017; 88:978-984. doi: 10.1016/j.biopha.2017.01.127

25) Sannikova EP, Bulushova NV, Cheperegin SE, Gubaydullin II, Chestukhina GG, Ryabichenko VV, Zalunin IA, Kotlova EK, Konstantinova GE, Kubasova TS, Shtil AA, Pokrovsky VS, Yarotsky SV, Efremov BD, Kozlov DG. The Modified Heparin-Binding L-Asparaginase of Wolinella succinogenes / Mol Biotechnol. 2016;58(8-9):528-39. doi: 10.1007/s12033-016-9950-1

26) Pokrovsky VS, Kazanov MD, Dyakov IN, Pokrovskaya MV, Aleksandrova SS. Comparative immunogenicity and structural analysis of epitopes of different bacterial L-asparaginases / BMC Cancer. 2016; 11;16:89. doi: 10.1186/s12885-016-2125-4

27) Pokrovskaya MV, Aleksandrova SS, Pokrovsky VS, Veselovsky AV, Grishin DV, Abakumova OY, Podobed OV, Mishin AA, Zhdanov DD, Sokolov NN. Identification of functional regions in the Rhodospirillum rubrum L-asparaginase by site-directed mutagenesis / Mol Biotechnol. 2015;57(3):251-64. doi: 10.1007/s12033-014-9819-0

28) Morozova EA, Kulikova VV, Yashin DV, Anufrieva NV, Anisimova NY, Revtovich SV, Kotlov MI, Belyi YF, Pokrovsky VS, Demidkina TV. Kinetic Parameters and Cytotoxic Activity of Recombinant Methionine γ-Lyase from Clostridium tetani, Clostridium sporogenes, Porphyromonas gingivalis and Citrobacter freundii / Acta Naturae. 2013; 5(3):92-8. doi: 10.32607/20758251-2013-5-3-92-98

29) Pokrovsky VS, Treshalina HM, Lukasheva EV, Sedakova LA, Medentzev AG, Arinbasarova AY, Berezov TT. Enzymatic properties and anticancer activity of L-lysine α-oxidase from Trichoderma cf. aureoviride Rifai BKMF-4268D / Anticancer Drugs. 2013;24(8):846-51. doi: 10.1097/CAD.0b013e328362fbe2.

30) Babich OO, Pokrovsky VS, Anisimova NY, Sokolov NN, Prosekov AY. Recombinant l-phenylalanine ammonia lyase from Rhodosporidium toruloides as a potential anticancer agent / Biotechnol Appl Biochem. 2013;60(3):316-22. doi: 10.1002/bab.1089

31) Pokrovskaya MV, Aleksandrova SS, Pokrovsky VS, Omeljanjuk NM, Borisova AA, Anisimova NY, Sokolov NN. Cloning, expression and characterization of the recombinant Yersinia pseudotuberculosis L-asparaginase / Protein Expr Purif. 2012 82(1):150-4. doi: 10.1016/j.pep.2011.12.005